New phytoplankton closed reference database

New phytoplankton closed reference database

One of Jonah’s advances in helping clients with metabarcoding was to develop a closed reference database for higher plants to aid in long-term continuity of data. In essence, we permanently assign a consensus sequence to a unique OTU ID. That way, we have...
Sequencing raptor diet

Sequencing raptor diet

On the diet side, we have reconstructed diets for a number of birds for clients. We’ve looked at what insects and plants prairie chickens eat. We’ve quantified the plants that geese in Alaska eat. We’ve looked at the insects and other invertebrates...
Is aquatic eDNA ready?

Is aquatic eDNA ready?

A short note here to share our thinking around the office and lab… Here, at Jonah Ventures we’ve spent a fair amount of resources working to understand how to apply next generation sequencing technology to aquatic environmental DNA. Speaking non-technically for...
An aid to help sample water

An aid to help sample water

We were running a pilot project the other day that required us to take 20 aquatic eDNA samples from the same site. The goal of the project is to look at species accumulation curves as we sample more and more water. The project requires pushing water through our...
Bird DNA in water

Bird DNA in water

We just finished analyzing ~100 stream eDNA samples for Nebraska DEQ. We ran these samples with 12S primers that amplify vertebrate DNA. The goal of the project was to look for DNA that might be helpful in determining the sources of fecal coliform bacteria in the...
Sequencing the Potomac River

Sequencing the Potomac River

The Potomac River starts small, at a place called Fairfax Stone. 300 miles later–as it passes by Washington DC and enters the Chesapeake Bay estuary, it’s very large. Along the way the Potomac does more than pick up water. It picks up nutrients from the...
Learning more about diet

Learning more about diet

Some of our scientific ignorances are so vast we do not even realize it. What animals eat is one of them. At Jonah Ventures, we’ve analyzed the diets of over 25 different species and every time we do, we learn something entirely new. But, continuing to learn new...
Parasites in animals

Parasites in animals

A lot of things come out with fecals. Not only is their DNA from animals ate and the animals themselves, but also DNA from parasites that infect the animals. Common parasites in the digestive system include nematodes, trematodes, and cestodes. Traditional techniques...
Fish environmental DNA

Fish environmental DNA

The technique of assessing the relative abundance of different organisms is one of the strengths of next generation sequencing. For one recent project, we assessed the relative abundance of different fish species across a number of streams in Nebraska by amplifying a...
Sampling organisms of Mississippi River

Sampling organisms of Mississippi River

Jonah Ventures is currently working with Cameron Thrash at Louisiana State University to quantify the organisms of the Mississippi River using eDNA. The project is an amazing effort on the part of volunteers that have rowed the entire length of the Mississippi,...