Improve Your Understanding of Plants

JonahPlant delivers insight into the health of plants

Our JonahPlant services answer questions about domestic and wild plants

By sequencing plant material or soil, we quantify pathogens and mutualistic microbes

We sequence the bacteria and fungi of plant microbiomes

JonahPlant helps quantify: 

  • Fungal pathogens such as Phythium or Fusarium

  • Bacterial pathogens such as Pseudomonas syringae or Ralstonia solanacearum

  • Microbial probiotic assemblages in leaves, roots, or seeds

 

An individual leaf can host this many bacterial taxa

Helping growers avoid major losses due to plant diseases has been incredibly rewarding. The potential to see what has so long been invisible is staggering.

Joe Craine, PhD

Co-Founder, Jonah Ventures

Our Next Generation Sequencing Process

1. Collect

Collect sample into barcoded vial.

Send to Jonah Ventures Lab.

2. Extract

We extract DNA from sample.

3. Amplify

Specific regions of DNA are amplified into amplicons using pairs of primers.

Each primer pair is specific to a broad taxonomic group.

4. Index

Short, unique segments of DNA are attached to each amplicon to index from which sample it originated.

5. Pool

Indexed amplicons from multiple samples are pooled together

6. Sequence

Amplicons are sequenced

7. Bioinformatics

Data from sequencer is bioinformatically processed to identify groups of similar sequences called OTUs.

8. Identify

OTUs are matched against a reference database to identify the species each OTU represents.

Example Data

Shown here are data on fungal abundance sequenced on plant leaves. In this case, leaves are macerated and the DNA extracted, amplified, and sequenced.

Taxa such as Olpidium brassicae, Botryosphaeriaceae species and Fusarium sp., for example, are all known to be plant pathogens and a potential indicator of developing fungal outbreak in these plants.

Our Standard Deliverables

b

OTU Sequences

Observed genetic sequences that map to each OTU

b

OTU Taxonomy

Details the species that have the sequences that comprise each OTU

OTU Table

Describes number of times the sequences for each OTU was read for each sample

Pricing

Orders of <25 samples require an additional fee of $150 for bioinformatics setup per taxonomic group

Discounts available for large orders. Please contact to discuss.

If providing extracted DNA, samples are discounted $25/sample.

Request your free quote today!

Types of measurements

Jonah Ventures
1600 Range Street Suite 201
Boulder, CO 80301
(785) 409-1655
email: info@jonahventures.com

Jonah Ventures