Frequently Asked Questions
You can purchase individual JonahWater Aquatic eDNA kits, JonahWater DNA Source-tracking kits, JonahAnimal Diet kits, or JonahAnimal Honey kits at our online store
For custom kits, bulk orders, and large order discounts, please contact us for a quote.
Our DNA kits should be stored at room temperature. There is no shelf life for them so it is fine to order multiple kits at once and use them as needed.
Samples should be sent to 5485 Conestoga Ct Ste 210 Boulder, CO 80301.
If you are sending samples to be analyzed for a custom set of primers please make sure to contact us before shipment.
We will not be able to receive samples on Saturday or Sunday. Please make sure that your samples arrive Monday-Friday so we can process them.
Small numbers of filters can simply be mailed in the provided blue bubble mailers. If you are shipping a large volume of samples they can be packaged in any box or envelope. There is no need for insulated shipping containers. We recommend using FedEx or UPS two-day shipping.
qPCR results will be returned within a few days to a week assuming we have an assay ready to go. If we need to order a new assay turnaround time will increase.
For anything NGS, it takes 4-6 weeks for data return because of the additional sample preparation necessary.
We can expedite services for an additional fee. Please contact us to discuss.
A link to access your results will be emailed to you when they are available. Return to JonahDNA.com at anytime to view or download.
In short, we include a spike-in of a known quantity of an ostrich DNA sequence to our samples in order to back-calculate the initial quantity of a template sequence in a sample. To learn more about how quantitative metabarcoding works, you can read our white paper here.
As of Q4 2024, almost all sequencing will be conducted on Oxford Nanopore sequencers using Minion flow cells. Our internal research has showed that amplicon results are nearly indistinguishable when sequenced on Illumina Miseq and Oxford Nanopore Minion. The transition to Oxford Nanopore sequencing will allow us to turnaround samples more quickly and provide greater sequencing depth. In the future, we will also likely begin to offer sequencing longer amplicons to provide greater taxonomic resolution. You can read more about our Illumina vs Nanopore comparison study here.