Improve Your Understanding of Animals

JonahAnimal delivers insight into animal diet

Our JonahAnimal services answer questions about wildlife, livestock, and pests.

By sequencing animal fecal material, we quantify diet, parasites, and gut microbes.

We also sequence honey and pollen to quantify plant use by pollinators

JonahAnimal gives you the capacity to measure diets for

  • Herbivores such as bison, elk, cattle, and rodents

  • Omnivores such as pigs and bears

  • Predators such as fox, hawks, and bats

 

Species Studied So Far

DNA metabarcoding is a monumental leap forward in our ability to understand what animals are eating. The first time I received data back from our bison, I remember thinking, ‘This changes everything.’

Joe Craine, PhD

Co-Founder, Jonah Ventures

Our Next Generation Sequencing Process

1. Collect

Collect sample into barcoded vial.

Send to Jonah Ventures Lab.

2. Extract

We extract DNA from sample.

3. Amplify

Specific regions of DNA are amplified into amplicons using pairs of primers.

Each primer pair is specific to a broad taxonomic group.

4. Index

Short, unique segments of DNA are attached to each amplicon to index from which sample it originated.

5. Pool

Indexed amplicons from multiple samples are pooled together

6. Sequence

Amplicons are sequenced

7. Bioinformatics

Data from sequencer is bioinformatically processed to identify groups of similar sequences called OTUs.

8. Identify

OTUs are matched against a reference database to identify the species each OTU represents.

Example Data

Shown here are data on the proportions of different species in bison diet taken over three years from the same herd in South Dakota using our metabarcoding techniques. Top three diet items shown. Across the three years, bison routinely consumed high proportions of cool-season grasses in the spring and fall, while maximizing intake of protein from legumes in the summer.

Whether it is for seeing a time course of diet or comparing across populations or species, we can reconstruct the diets of herbivores, omnivores, or predators using our JonahAnimal services.

Our Standard Deliverables

b

OTU Sequences

Observed genetic sequences that map to each OTU

b

OTU Taxonomy

Details the species that have the sequences that comprise each OTU

OTU Table

Describes number of times the sequences for each OTU was read for each sample

Pricing

*$5 handling fee for honey due to extra processing steps

Orders of <25 samples require an additional fee of $150 for bioinformatics setup per taxonomic group

Discounts available for large orders. Please contact to discuss.

If providing extracted DNA, samples are discounted $25/sample.

Request your free quote today!

Types of measurements

Jonah Ventures
1600 Range Street Suite 201
Boulder, CO 80301
(785) 409-1655
email: info@jonahventures.com

Jonah Ventures